A comprehensive collection of essential and easily reproducible techniques currently used by leading researchers to manipulate RNA molecules and to inactivate gene expression in vivo. The methods range from theoretical design to clinical application and include the synthesis and assay of ribozymes, as well as their use both in vitro and in vivo. Each method is presented by an expert with substantial hands-on experience and in far greater detail than is to be generally found in the research literature. The book will be indispensable not only to those workers new to the field, but also to experienced researchers who want to expand their range of expertise.

 

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